Full Time
Florida
Posted 1 year ago

Cell Culture Biologist

The Biotechnology Discovery Research (BioTDR) organization at company supports the discovery and optimization of a wide array of novel therapeutic modalities including proteins, peptides, fusions, and conjugates. The Lead Optimization Core group within BioTDR is seeking a hardworking individual to join our diverse, interdisciplinary team as a protein expression scientist. We are looking for a candidate who will contribute to leading the generation of novel recombinant proteins using our ground breaking mammalian and microbial expression platforms, to support our innovative biotherapeutic pipeline. Do you have a strong technical understanding of protein production platforms along with excellent communication and collaborative skills? This role will help to accelerate our next-generation therapeutics in this fast-paced and dynamic environment. We want you on our team! Apply today!

Responsibilities:

Expression of recombinant proteins using mammalian cell hosts, primarily CHO and HEK293 cells in both transient and stable fed-batch production systems.
Transfection of mammalian cells with plasmid DNA, mRNA and/or protein.
Determination of recombinant protein titers and product quality analysis by ProA-HPLC, Octet, SDS-PAGE, Western blotting and CE-SDS.
Close teamwork and with colleagues in alleviating protein expression bottlenecks.
Cell culture technology development and evaluation through experimental DOE design, execution, and analysis of results.
Document experimental design and results in electronic laboratory notebooks and generate technical reports and protocols.
Communication of experimental findings to team members and presentation of results at cross-site team meetings.

Basic Requirements:

Bachelor’s Degree (with 4 years of scientific laboratory experience) or Master’s Degree (with 2 years of laboratory experience) in Cell Biology, Molecular Biology, Biochemistry, Bioengineering, or a related subject area

Additional Preferences:

Highly organized with excellent written and verbal communication skills and ability to work in a fast-paced environment.
Experience in transient recombinant protein production using CHO or HEK293 mammalian expression systems in a shake flask or bioreactor system.
Stable cell line generation and mammalian cell bank preparation.
Design of expression plasmid constructs.
Cell line engineering experience through application of the CRISPR-Cas9 gene editing technology.
Characterization of purified proteins by SDS-PAGE, CE-SDS, ELISA and Western Blot analysis.
Technical understanding of fluorescence-activated cell sorting (FACS) analysis.
Experience with molecular and experimental design software such as Benchling and JMP.

To apply for this job please visit orbiterrecruiting.com.